She is the only female racer in the Wacky Races lineup (not that she minds), driving a pink feminine car known as The Compact Pussycat which has personal grooming facilities that would sometimes backfire on other racers, such as shampoo foam hitting their faces. She always has time to relax and worry about her looks, because her car is like a beauty parlor. Penelope Pitstop also has a habit of holding her arm out in the breeze to dry newly applied nail polish, which the others mistake for a turn signal (this habit once sent the Roaring Plenty into a cactus).
Peter Perfect had a crush on her, a feeling that was returned, and always tried to help her; in "Dash to Delaware", they almost end up married. She later has two children by him, Parker and Piper Perfect. She also has a sister, Pandora Pitstop.
Unlike other drivers, Penelope rarely was targeted by the other racers (except Dick Dastardly of course), as it seems they also liked and tried to help her as Peter did. Penny was always thankful for their assistance and was perhaps the most peaceful racer on the track, though she twice gave the Creepy Coupe's dragon and serpent a good bashing with her umbrella. In the 2017 version, she eventually shows martial arts skills.
Penelope also had her own cartoon series, The Perils of Penelope Pitstop, which also featured her rescuers the Ant Hill Mob. Her sworn enemy is the Hooded Claw, (who is unknown to her, her own guardian, Sylvester Sneekly). She wore a slight alteration to her Races wardrobe.
Penelope is an attractive southern belle. Although she is blind to the fact her guardian is her arch nemesis in disguise, she is very smart and often manages to get herself out of trouble before her true guardians the Ant Hill Mob can get to her in time.
In Wacky Races, Penelope has long blond hair in a full fringe. She wears a purple racer helmet with goggles. She also wears a dark pink rider jacket with a purple turtleneck and purple gloves, pink skirt with red pants underneath and white boots. In The Perils of Penelope Pitstop, Penelope's eyes resemble those of Daphne Blake, and despite having white sclerae, she wears red lipstick. Her helmet is replaced by a magenta pilot helmet with matching white goggles. She now wears a hot pink long-sleeved jacket with a matching belt and long magenta scarf, which is hanging loose on the right side. Even though she still wears her red pants and white boots from Wacky Races, her gloves are now white.
- Penelope's car, the Compact Pussycat, and the Crimson Haybaler are the only cars not named during the opening titles.
- The Compact Pussycat never appeared in The Perils of Penelope Pistop; it was replaced by a green limousine-like vehicle.
- The Hooded Claw does change between his true identity twice in front of Penelope. The first time wasn't as obvious as he was bending down behind a desk, the second time she refuses to believe the truth and finds it an insult to her guardian.
- In the 2017 Wacky Races series, she has a twin sister named Pandora.
- In the first episode of The Perils of Penelope Pitstop, Penelope reveals she had won the Miss Banana Queen award. There is a real Latin American beauty contest, allowing only international participants from banana-producing or -consuming countries since 1985.
- The comic book series Wacky Raceland says she had lived in Aegina, Greece, but does not identify her as a native Greek.
- Penelope holds the distinction of being the winner of the last Wacky Race from the classic series, "The Carlsbad or Bust Bash".
- Penelope is referenced in the 2020 CGI animated film Scoob! with a perfume called Pitstop from "Penelope" and a Penelope Pitstop arcade cabinet in the abandoned Funland amusement park in Romania.
- In the 2017 Reboot, she's the only racer who views Dick Dastardly as a friend.
- Janet Waldo - Wacky Races, The Perils of Penelope Pitstop, Yogi's Treasure Hunt, Wacky Races Starring Dastardly and Muttley
- Kath Soucie - Wacky Races Forever
- Nicole Parker - Wacky Races (2017)